Hieff UNICON® Universal Blue qPCR SYBR Master Mix-1 mL

Hieff UNICON® Universal Blue qPCR SYBR Green Master Mix is ​​a pre-solution for 2× real-time quantitative PCR amplification. It has the characteristics of high sensitivity and high specificity. It is blue in color and has the function of sample addition and tracing. The core component, Hieff UNICON® Taq DNA polymerase, uses antibody hot start, which can effectively inhibit non-specific amplification caused by primer annealing during sample preparation. At the same time, the formula adds factors that improve the amplification efficiency of PCR reactions and promote factors that balance the amplification of genes with different GC contents (30~70%), so that quantitative PCR can obtain a good linear relationship in a wide quantitative range.

This product contains a special ROX Passive Reference Dye, which is suitable for all qPCR instruments. There is no need to adjust the ROX concentration on different instruments. You only need to add primers and template when preparing the reaction system to perform amplification.